Chitinophaga fulva sp. nov., isolated from forest soil

초록

An aerobic, Gram-stain-negative, oxidase- and catalase-positive, non-motile, non-spore-forming, rod-shaped and yellow-coloured bacterium designated strain G-6-1-13(T) was isolated from Gwanggyo mountain forest soil. Strain G-6-1-13(T) could grow at 15-40 degrees C (optimum, 20-32 degrees C), pH 4.5-10.5 (optimum, pH 6.0-9.0), at 2% (w/v) NaCl concentration, and produced flexirubin-type pigments. Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain G-6-1-13(T) formed a lineage within the genus Chitinophaga that was distinct from other species of the genus. Closest member was Chitinophaga varians 10-7 W-9003(T) (98.6% sequence similarity) followed by C. eiseniae DSM 22224(T) (98.4 %), C. qingshengii JN246(T) (97.6%) and C. terrae KP01(T) (97.4%). The major cellular fatty acids were iso-C-15:0, C-16:1 omega 5c, and summed feature 3 (iso-C-15:0 2-OH and/or C-16:1 omega 6c). MK-7 was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content of strain G-6-1-13(T) was 48.7 mol%. Average nucleotide identity and in silico DNA-DNA hybridization were below the species threshold. On the basis of phenotypic, genotypic, phylogenetic and chemotaxonomic characterization, G-6-1-13(T) represents a novel species in the genus Chitinophaga, for which the name Chitinophaga fulva sp. nov. is proposed. The type strain is G-6-1-13(T) (=KACC 21624(T)=NBRC 114361(T)).

키워드