Evaluation of DNA and cell damage induced by intense pulsed light in Pseudomonas aeruginosa; Intense pulsed light에 의해 유도된 Pseudomonas aeruginosa의 DNA 및 세포 손상에 대한 평가

초록

The present study aimed to evaluate the bactericidal effect of intense pulsed light (IPL) on Pseudomonas aeruginosa and DNA and cell damage induced by IPL treatment. Microbial inactivation by IPL improved with higher voltages and prolonged exposure, showing a reduction of approximately 6.0 log for 90 s at 600 V and 60 s at 800 V. The temperature changes during IPL treatment on P. aeruginosa cells were 1.05oC for 90 s at 600 V and 1.57oC for 60 s at 800 V. IPL treatment resulted in the accumulation of double-strand breaks (DSBs), single-strand breaks (SSBs), and cyclobutane pyrimidine dimers (CPDs) in the DNA of P. aeruginosa cells. Transmission electron microscopy (TEM) confirmed that the structure of untreated cells was maintained, whereas disrupted membranes and ruptured internal tissues were observed in treated cells. These findings suggest that IPL treatment effectively inactivates P. aeruginosa and has potential applications in food sterilization. ©The Korean Society of Food Science and Technology.

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