Green chemistry approach for silver nanoparticles synthesis from Halimeda macroloba and their potential medical and environmental applications

초록

This study aims to synthesize silver nanoparticles (AgNPs) using Halimeda macroloba extract and assess their anticancer and photocatalytic properties. Ultraviolet–visible spectrophotometry (UV–visible), Fourier transform infrared (FT-IR) spectroscopy, Transmission electron microscope (TEM), scanning electron microscope-energy-dispersive spectroscopy (SEM–EDX), X-ray photoelectron spectroscopy (XPS), and particle-size distribution (PSD) were used to characterize the synthesized AgNPs. The green-synthesized AgNPs to be spherical in shape with a size of about 50–100 nm. The half-maximal inhibitory concentration (IC<inf>50</inf>) for AgNPs was determined to be 89.5 g/mL against the human hepatoma cell line (Huh-7). Fluorescent microscopy was used to evaluate the morphological alterations of Huh-7 cells stained with acridine orange/ethidium bromide (AO/EtBr), 2′-7′dichlorofluorescin diacetate (DCFH-DA), and rhodamine 123. Meanwhile, AgNPs exhibited 91.35% photocatalytic activity against methylene blue (MB) after 100 min of exposure to sunlight. Therefore, AgNPs have a strong potential for usage in anticancer activities and photocatalytic organic pollutant destruction. © 2023, King Abdulaziz City for Science and Technology.

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