Antibacterial activity of silver nanoparticles phytosynthesized from Glochidion candolleanum leaves

초록

In the present study, Glochidion candolleanum (Wight & Arn.) Chakrab. & M. Gangop. leaf (GCL)-ethyl acetate extract was used for the phytosynthesis of silver nanoparticles (AgNPs) and subjected to antibacterial assay with G(+ve) and G(-ve) bacteria. The GCL center dot AgNPs synthesized were characterized through various spectroscopic and microscopic analyses, while the phytochemical compounds was analyzed by GC-MS. The discrete GCL center dot AgNPs formed were micron-scale range. TEM images of AgNPs reveal that nanoparticles were spherical and ellipsoidal in shape, dispersed uniformly and capped with secondary material of approximate to 10 nm thickness. A maximum inhibition zone of 12.2 mm diameter was observed against Salmonella enterica followed by 11.8 mm diameter against Pseudomonas aeruginosa. GC-MS analysis revealed the occurrence of bioactive phytocompounds in GCL extract, hexamethylcyclotrisiloxane, 2,4,6-cycloheptatrien, benzenepropanoic acid, 2-ethylacridine, benzo[h]quinoline, methyltris(trimethylsiloxy)silane and 5-methyl-2-phenylindolizine. The findings suggest that GCL could be used for developing antibacterial AgNPs against pathogenic bacteria.

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