다양한 채취 방법에 따른 구강미생물의 정량적 비교연구: 예비연구

초록

Objectives: This study aimed to compare each strain’s number of microorganisms found in oral samples collected using various collection methods. Methods: Twenty-two adults aged 40 and above participated in the study. Oral samples were col lected from subjects using three different methods (stimulated saliva, oral biofilm, and calculus),and the collected samples were analyzed using the multiplex real-time Polymerase chain reaction (PCR) method. Results: The study included 22 subjects (2 men, 20 women) with an age range of 40-75 years. Healthy oral condition was observed in 10 subjects, while the remaining 12 had periodontitis. The saliva and biofilm collection methods for oral microorganisms detected Porphyromonas gingivalis(Pg), Tannerella forsynthesis (Tf), and Fusobacterium nucleatum (Fn), which are the causative bac teria of periodontal disease, more effectively compared with the calculus group. In addition, the sa liva group showed a better ability to detect Streptococcus mutans (Sm) which causes dental caries, compared with the biofilm and calculus groups. Comparisons based on the presence or absence of periodontitis and the collection method revealed a statistically significant difference in the number of oral microorganisms only in case of Sm strain. Conclusions: The frequency of expression of certain strains varies according to the method of col lection of oral microorganisms. Further, the saliva and biofilm methods of collecting oral microor ganisms are more suitable for quantitative analysis of bacteria causing periodontal disease.

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