Chitosan-stabilized platinum nanoparticles induce apoptotic cell death in breast cancer cells

초록

In the present work, chitosan from shrimp shells was used to synthesize chitosan-stabilized platinum nanoparticles for anticancer activities against breast cancer cell lines (MDA-MB-231). The synthesized chitosan-stabilized platinum nanoparticles were validated by spectroscopic examination. FT-IR measurements reveal the weak electrostatic interaction between the NH2 and OH groups of chitosan during the formation of platinum nanoparticles. The obtained chitosan-stabilized platinum nanoparticles are flower-shaped with enhanced colloidal stability, and their size spans from 75 to 150 nm with monodispersity. In addition, after 24 h of incubation, the chitosan-stabilized platinum nanoparticles kill breast cancer cells at a half-maximal inhibitory dose of 12 +/- 2.14 mu g mL(-1). In addition, the biocompatibility of the chitosan-stabilized platinum nanoparticle with human embryonic kidney cells was demonstrated (HEK-293). In addition, fluorescence microscopy reveals that chromatin condensation and nuclear disintegration induce apoptosis in breast cancer cells. Our findings indicate that chitosan-stabilized platinum nanoparticles can effectively kill breast cancer cells by rapidly triggering cytotoxicity and apoptosis.

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