Molecularly Imprinted Chalcone-Branched Polyimide-Based Chemosensors with Stripe Nanopatterns for the Detection of Melittin

초록

Inthis study, a chalcone-branched polyimide (CB-PI) was synthesizedby the Steglich esterification reaction for selective recognitionof the toxic peptide melittin (MEL). MEL was immobilized on a nanopatternedpoly(dimethylsiloxane) (PDMS) mold using a conventional surface modificationtechnique to increase binding sites. A stripe-nanopatterned thin CB-PIfilm was formed on a quartz crystal (QC) substrate by simultaneouslyperforming microcontact printing and ultraviolet (UV) light dimerizationusing a MEL-immobilized mold. The surface morphology changes and dimensionsof the molecularly imprinted polymer (MIP) films with stripe nanopatterns(S-MIP) were analyzed using scanning electron microscopy (SEM) andatomic force microscopy (AFM). The sensing signals (Delta f and Q (e)) of the S-MIP sensorwere investigated upon adsorption in a 100-mu L dilute plasmasolution containing 30 mu g/mL MEL, and its reproducibility, reuse,stability, and durability were investigated. The S-MIP sensor showedhigh sensitivity (5.49 mL/mg) and coefficient of determination (R (2) = 0.999), and the detection limit (LOD) andthe quantification limit (LOQ) were determined as 0.3 and 1.1 mu g/mL,respectively. In addition, the selectivity coefficients (k*) calculated from the selectivity tests were 2.7-5.7, 2.1-4.3,and 2.8-4.6 for bovine serum albumin (BSA), immunoglobulinG (IgG), and apamin (APA), respectively. Our results indicate thatthe nanopatterned MIP sensors based on CB-PI demonstrate great potentialas a sensing tool for the quantitative analysis of biomolecules.

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