Increased lamina propria B cells play roles in fructose-induced hypertension of Dahl salt-sensitive rats

초록

Aims: Although the immune system participates in the development of hypertension, the proportional contributions of distinct immune cells remain poorly understood. With the development of transcriptomics, we can profile the transcriptomes of individual immune cells and assess the relative contribution of each immune cell to the development of hypertension. So, we tested the hypothesis that increased lamina propria B cells play roles in fructose-induced hypertension of Dahl salt-sensitive (SS) rats. Materials and methods: Eight-week-old Dahl SS and Dahl salt-resistant (SR) male rats were divided into four groups; each group received either tap water (TW) or a 20 % fructose solution (HFS) for 4 weeks. Systolic blood pressure was measured using the tail-cuff method. Single-cell RNA sequencing (scRNA-seq) analysis was performed on lamina propria (LP) cells and peripheral blood mononuclear cells (PBMCs) obtained from the SS and SR rats subjected to either TW or HFS. Key findings: Results revealed that high-fructose intake induced hypertension in the SS rats but not in the SR rats. It also increased B cells in LPs but not in PBMCs of the SS rats; their subsets showed increased follicular and na & iuml;ve B cells. Increased lamina propria B cells play roles in fructose-induced hypertension of SS rats. Significance: This finding suggest that targeting B cells could be a potential strategy to mitigate high blood pressure in fructose-induced hypertension.

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