C2C12 근육모세포와 전기자극에 따른 FNDC5/irisin pathway 기전 연구

초록

The tThe this study, we attempted to investigate the effects of EPS (Electrical Pulse Stimulation) the C2C12 cell line composed of mouse skeletal muscle cells. The experimental group was divided into -Electrical pulses stimulation (CON), Low Electrical pulses stimulation (L-EPS), High Electrical pulses stimulation (H-EPS), electrical impulse was introduced to the differentiated C2C12 myotubes for four days under the condition of high intensity 14V/mm, 50Hz, 1ms, low intensity 0.3 V/mm, 1.0 Hz, and 4.0 ms. Then, the C2C12 cell’s morphological change was analyzed along with changes in the cell length, and western blot analysis was conducted on the protein expressed from the C2C12 cell. The C2C12 cell line, composed of the mouse skeletal muscle cells, was stimulated through EPS after its differentiation. The expression of PPAR-γ was significantly higher in the H-EPS group, and the results of PGC-1α, FNDC5, and irisin showed statistically significant differences between each group, and the L-EPS group treated with electrical stimulation compared to the CON group, the H-EPS group showed significantly higher activity. It is thought that the increase in muscle PGC-1α, FNDC 5, and Irisin, which are browning factors for fat, after electrical stimulation will lead to an increase in UCP-1, PPAR-γ, PRDC 16, and FABP-4 in fat tissue, that is, the intensity of exercise. Accordingly, by presenting positive results in the muscle factor among fat browning factors, based on this, it is possible to establish factors such as increased utilization of energy metabolism, anti-obesity, and metabolic syndrome through research on physiological mechanisms in relation to various exercise intensities and methods.

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