Characteristics of endophytic bacteria in Cnidium officinale Makino from the Republic of Korea

초록

This research was carried out to investigate the characteristics of endophytic bacteria from the medicinal plant Cnidium officinale Makino. The plants were collected from four different fields of Bonghwa, Jeongseon, Jecheon and Taebaek in South Korea. Their endophytic bacte- ria and DNA were extracted and analyzed. As a result of the culture, eight species of culturable endophytic bacteria could be isolated, and Enterobacter ludwigii and Pantoea agglomerans were identified as the main culturable endophytic bacteria. In addition, next generation se- quencing (NGS) analysis was performed to analyze unculturable endophytic bacteria, and five orders including Rhizobiales and Streptomycetales were dominant. As a result of beta- diversity analysis, there was no significant difference (p > 0.05) between each region. Three modules were identified through network analysis, and eight connectors with high among- module connectivity were confirmed. Among them, the relative frequency of Streptomyces sp. is the highest, and it is considered the most important endophytic bacteria in Cnidium. Function prediction was performed with phylogenetic investigation of communities by recon- struction of unobserved states 2 (PICRUSt 2), and module 1 was highly related to the bacterial secretion system. On the other hand, module 2 and module 3 were highly related to plant- pathogen interaction and biosynthesis of siderophore. Therefore, in this study, we suggest that Streptomyces sp. play a key role in maintaining health and growth of Cnidium.

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