C2C12근세포에 Dexamethasone처치 후 EPS 및 NO3가 근수축인자와 GLUT-4에 미치는 영향

초록

This study, we attempted to investigate the effects of EPS (Electrical Pulse Stimulation) and Nitrate on muscle contraction and contraction phenomenon after inducing muscle loss by treating Dexamethasone using the C2C12 cell line composed of mouse skeletal muscle cells. The experimental group was divided into CON Group, -E/N0.5 Group, -E/N1.0 Group, +E/-N Group, +E/N0.5 Group, +E/N1.0 Group. For the EPS experiment, electrical impulse was introduced to the differentiated C2C12 myotubes for four days under the condition of 0.3 V/mm, 1.0 Hz, and 4.0 ms(+E/ Group). On the other hand, for the NO experiment, the Nitrate was treated with the differentiated C2C12 myotubes with final concentration of 0 mM, 0.5 mM and 1.0 mM. Then, this was cultured for four days(/+N Group). After the treatment, the results were derived from the Nitrate dosage that was generated and secreted from the C2C12 cells. Then, the C2C12 cell’s morphological change was analyzed along with changes in the cell length, and western blot analysis was conducted on the protein expressed from the C2C12 cell. The C2C12 cell line, composed of the mouse skeletal muscle cells, was stimulated through EPS and EPS+NO after its differentiation. The occurrence of muscle contraction phenomenon is caused by increased expression of eNOS and nNOS and AMPK, JNK, AKT, Glut4 increase the influx of glucose into muscle cells, thereby promoting mitochondrial fatty acid β-oxidation and reducing fatty acid synthesis. It is thought that this may have induced an increase in muscle contraction due to the simultaneous stimulation of EPS and NO3. Therefore, the results of this study suggest the possibility that exercise and supplementation with plant-based protein rather than animal-based protein will increase exercise performance, and in the future, the signal transduction system related to muscle contraction can be improved by using plant-based protein, which causes significantly less toxicity. It is believed that by conducting research on and interaction, it will have a positive effect on increasing muscle contraction and exercise performance in athletes as well as non-trainers.

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