Sustainable biodiesel production from slaughter byproduct fat using zirconia-lipase cluster

초록

Refined slaughter byproduct fat is obtained through the thermal and mechanical processing of animal waste generated during slaughtering and meat processing. The utilization of these non-edible fats and oils as feedstocks for biodiesel production is highly beneficial since it does not compete with the food industry and contributes to global waste management by reducing waste. This study aimed to stabilize lipase from Pseudomonas fluorescens by immobilizing it with zirconia to form a zirconia-lipase cluster and applying it in the transesterification of waste fat from refined slaughter byproduct to produce biodiesel in a solvent-free medium. The immobilized lipase demonstrated hyperactivated hydrolysis activity, with 125 % higher specific hydrolysis activity than free lipase did, and 54 % of the initial activity of the immobilized lipase was retained after 100 days of storage at room temperature. The kinetic parameters from the kinetic study indicated that compared with free lipase, zirconialipase clusters significantly improved substrate affinity and catalytic efficiency on the basis of its lower km and higher Vmax.Km-1. Additionally, in a solvent-free transesterification system, the zirconia-lipase clusters achieved a biodiesel yield that was 4.16 times greater than that of the free lipase. To the best of our knowledge, this is the first report on the conversion of refined slaughter byproduct fat into biodiesel via a solvent-free reaction catalyzed by hyperactive zirconia-lipase clusters.

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