Compound K-enriched Korean red ginseng prevents lung cancer progression by targeting cancer cells and fibroblasts

초록

Background: Despite the efficacy of anticancer drugs, patients frequently experience relapse, metastasis, andresistance. A promising therapeutic approach not only targets cancer cell growth but also modulates cancerassociatedfibroblasts, which support malignancies. Compound K (CK), a metabolite derived from red ginseng,has demonstrated anticancer properties. Recently, we developed a CK-enriched red ginseng extract (CKP) andexplored its potential to suppress lung cancer by inhibiting cancer cell proliferation and inactivating fibroblasts. Methods: To evaluate the in vitro efficacy of CKP in inhibiting lung cancer cell proliferation, MTT and colonyformation assays were performed. The apoptotic effects of CKP on lung cancer cells were assessed using Westernblot and flow cytometry. Furthermore, the ability of CKP to inhibit TGF β1-induced migration of cancer cells wasinvestigated through Western blot, RT-PCR, and a wound healing assay. Additionally, the impact of CKP on lungfibroblast inactivation was examined via Western blot and RT-PCR analysis. For in vivo experiments, a xenograftmodel was utilized, incorporating a combination of lung cancer cells and lung fibroblasts in xenografts. Results: CKP significantly reduced the proliferation and invasiveness of TGF-β1-stimulated A549 cells, demonstratingits potential to inactivate lung fibroblasts. Additionally, CKP inhibited the secretion of cytokines, such asinterleukin-6, interleukin-8, and TGF-β1, by activated fibroblasts. In vivo, CKP markedly inhibited tumor growthin the xenograft model. Conclusion: In conclusion, CKP effectively induced apoptosis in lung cancer cells, suppressed metastasis, andinactivated fibroblasts, thereby preventing cancer invasion and reducing extracellular matrix production,highlighting its potential as a novel anticancer agent.

키워드