Lactobacillus attenuates progression of nonalcoholic fatty liver disease by lowering cholesterol and steatosis

초록

Background/Aims: Nonalcoholic fatty liver disease (NAFLD) is closely related to gut-microbiome. There is a paucity of research on which strains of gut microbiota affect the progression of NAFLD. This study explored the NAFLD-associated microbiome in humans and the role of Lactobacillus in the progression of NAFLD in mice. Methods: The gut microbiome was analyzed via next-generation sequencing in healthy people (n=37) and NAFLD patients with elevated liver enzymes (n=57). Six-week-old male C57BL/6J mice were separated into six groups (n=10 per group; normal, Western, and four Western diet + strains [10(9) colony-forming units/g for 8 weeks; L. acidophilus, L. fermentum, L. paracasei, and L. plantarum]). Liver/body weight ratio, liver pathology, serum analysis, and metagenomics in the mice were examined. Results: Compared to healthy subjects (1.6 +/- 4.3), NAFLD patients showed an elevated Firmicutes/Bacteroidetes ratio (25.0 +/- 29.0) and a reduced composition of Akkermansia and L. murinus (P<0.05). In the animal experiment, L. acidophilus group was associated with a significant reduction in liver/body weight ratio (5.5 +/- 0.4) compared to the Western group (6.2 +/- 0.6) (P<0.05). L. acidophilus (41.0 +/- 8.6), L. fermentum (44.3 +/- 12.6), and L. plantarum (39.0 +/- 7.6) groups showed decreased cholesterol levels compared to the Western group (85.7 +/- 8.6) (P<0.05). In comparison of steatosis, L. acidophilus (1.9 +/- 0.6), L. plantarum (2.4 +/- 0.7), and L. paracasei (2.0 +/- 0.9) groups showed significant improvement of steatosis compared to the Western group (2.6 +/- 0.5) (P<0.05). Conclusions: Ingestion of Lactobacillus, such as L. acidophilus, L. fermentum, and L. plantarum, ameliorates the progression of nonalcoholic steatosis by lowering cholesterol. The use of Lactobacillus can be considered as a useful strategy for the treatment of NAFLD.

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