Imperatorin reduces thrombus formation by inhibiting platelet function and phosphoproteins

초록

Platelets, disc shaped fragments of megakaryocytes, are involved in blood coagulation and aggregation, however, platelet can cause unexpected blood clots simultaneously. Therefore, to prevent circulatory disorders by platelets, proper platelet regulation is essential. We checked antiplatelet effects of imperatorin on platelet aggregation, calcium release and integrin αIIb/β<inf>3</inf> activation. We observed that imperatorin inhibited platelet aggregation, calcium release and glycoprotein IIb/IIIa (αIIb/β<inf>3</inf>) activation and regulated its signaling molecules. Imperatorin elevated the phosphorylation of Akt, glycogen synthase kinase-3α/ β (GSK-3α/β), spleen tyrosine kinase, and phospholipase C<inf>γ2</inf> and vasodilator stimulated phosphoprotein. In addition, imperatorin effectively delayed thrombin-induced clot contraction time. To summarize the results, imperatorin shows strong antiplatelet effects and delayed thrombin-induced clot retraction. These results lead us to expect that imperatorin may be helpful in treating cardiovascular disease. © The Korean Society for Applied Biological Chemistry 2024.

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