Hymenobacter busanensissp. nov., radiation-resistant species isolated from soil in South Korea

초록

Two bacterial strains designated as MA3(T)and BT182 were isolated from a soil sample in South Korea. Cells of the two strains were Gram-stain-negative, non-motile, rod-shaped and formed red colonies on R2A agar at 25 degrees C. The 16S rRNA genes of the two strains shared a sequence similarity of 99.8%. Both strains shared the highest 16S rRNA gene similarity of 96.8% withHymenobacter edaphiNL(T), followed byHymenobacter paludisKBP-30(T)(96.3%),Hymenobacter coalescensWW84(T)(96.3%) andHymenobacter gummosusANT-18(T)(96.3%). Growth was observed at 15-37 degrees C (optimum 30 degrees C), pH 6-8 (optimum pH 7) and in the presence up to 1% NaCl. The genome size of strains MA3(T)and BT182 is 4.9 Mb and 4.8 Mb, respectively. The genomic G + C content of both strains is 62.0 mol%. The main polar lipid of the strains was phosphatidylethanolamine, the only respiratory quinone detected was menaquinone-7 and the major fatty acids were anteiso-C-15:0, iso-C-15:0, summed feature 4 (iso-C17:1I/anteiso-C17:1B) and summed feature 3 (C-16:1 omega 6c/C-16:1 omega 7c), supporting the affiliation of these strains with the genusHymenobacter. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, strains MA3(T)and BT182 represent a novel species of the genusHymenobacter, for which the nameHymenobacter busanensisis proposed. The type strain is MA3(T)(= KCTC 72631(T) = NBRC 114193(T)).

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