Effects of bone morphogenetic protein-2 and activated platelet-rich plasma on canine chondrocytes in monolayer cultures

초록

This study evaluated the effects of bone morphogenetic protein-2 (BMP-2) and platelet-rich plasma (PRP) on canine chondrocytes under inflammatory conditions. Four groups were tested: control, BMP, PRP, and PRP+BMP. Inflammatory conditions were induced using lipopolysaccharide. Realtime polymerase chain reaction (PCR) was performed to measure the expression of COL1A1, COL2A1, interleukin (IL)-1β, and osteocalcin, and a cell proliferation assay was conducted. Cell proliferation assays showed significant increases in both PRP and PRP+BMP groups, with no significant difference between them. In the real-time PCR, the BMP group showed no change in COL1A1 expression compared to the control group, but a significant increase in COL2A1 expression was observed. In contrast, the PRP and PRP+BMP groups showed significant decreases in both COL1A1 and COL2A1 expression. IL-1β expression was significantly decreased in the PRP and PRP+BMP groups, but no differences were observed in the BMP group compared to the control group. Regarding osteocalcin mRNA expression, no significant difference was observed between the control and BMP groups, but significant decreases were identified in the PRP and PRP+PRP groups. In conclusion, PRP enhanced cell proliferation and exhibited anti-inflammatory effects on chondrocytes, while BMP-2 increased COL2A1 gene expression. Interestingly, BMP-2 did not increase osteocalcin gene expression, which is associated with the osteoblast differentiation of stem cells, and the PRP groups exhibited inhibited osteocalcin gene expression under inflammatory conditions.

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