Extracellular vesicles derived from fibroblasts promote wound healing by optimizing fibroblast and endothelial cellular functions

초록

Extracellular vesicles (EVs) have been exhibited as promising candidates for delivering endogenous therapeutic cargos for regenerative therapies. The fibroblasts could a candidate source cells for EVs and investigating their therapeutic effects in wound healing. Here we demonstrated the isolation and characterization fibroblast (L929 cell line) derived EVs (L929-EVs). Furthermore, L929-EVs treatment showed pro-wound healing effects in vitro by enhancing proliferation, migration, and scarless wound healing related genes in fibroblast cells. L929-EVs treatment also enhanced the migration and tube formation of endothelial cells. The combination of L929-EVs with fibrin glue accelerated the wound healing in the skin wound mouse model by enhancing collagen formation, collagen maturation, and blood vessels in the wounded skin. The role of fibroblast derived EVs in the wound healing could be an important phenomena and this fibroblast derived EVs can be harnessed for wound healing therapies.

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