Genomic and Pathological Characterization of Acute Hepatopancreatic Necrosis Disease (AHPND)-Associated Natural Mutant Vibrio parahaemolyticus Isolated from Penaeus vannamei Cultured in Korea

초록

Simple Summary: Acute hepatopancreatic necrosis disease (AHPND) is a plasmid-encoded PirA/B toxin mediated bacterial disease that causes significant economic losses to the global shrimp industry. Since the first AHPND outbreak of Penaeus vannamei cultures in Korea in 2016, AHPND-associated Vibrio parahaemolyticus (Vp(AHPND)) is still considered a major threat to the Korean shrimp industry. Although several diagnostic methods have been developed against AHPND, several concerns have been raised about the misdiagnosis of the pathogen due to the transferability and mutation of the plasmid. In this study, we report the emergence of a natural mutant Vp(AHPND) (pirA(-), pirB(+)), which was isolated from cultured P. vannamei in Korea, and also provide its detailed genomic and pathological characteristics. Acute hepatopancreatic necrosis disease (AHPND) is one of the most important diseases in the global shrimp industry. The emergence of mutant AHPND-associated V. parahaemolyticus (Vp(AHPND)) strains has raised concerns regarding potential misdiagnosis and unforeseen pathogenicity. In this study, we report the first emergence of a type II (pirA(-), pirB(+)) natural mutant, Vp(AHPND) (strain 20-082A3), isolated from cultured Penaeus vannamei in Korea. Phenotypic and genetic analyses revealed a close relationship between the mutant strain 20-082A3 and the virulent Korean Vp(AHPND) strain 19-021-D1, which caused an outbreak in 2019. Detailed sequence analysis of AHPND-associated plasmids showed that plasmid pVp_20-082A3B in strain 20-082A3 was almost identical (>99.9%) to that of strain 19-021-D1. Moreover, strains 20-082A3 and 19-021-D1 exhibited the same multilocus sequence type (ST 413) and serotype (O1:Un-typeable K-serogroup), suggesting that the mutant strain is closely related to and may have originated from the virulent strain 19-021-D1. Similar to previous reports on the natural mutant Vp(AHPND), strain 20-082A3 did not induce AHPND-related symptoms or cause mortality in the shrimp bioassay. The emergence of a mutant strain which is almost identical to the virulent Vp(AHPND) highlights the need for surveillance of the pathogen prevalent in Korea. Further investigations to elucidate the potential relationship between ST 413 and recent Korean Vp(AHPND) isolates are needed.

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