국내산 아로니아 열매 추출물의 항산화 활성 및 A549 세포에 대한 세포독성 연구

초록

The extracts of aronia (Aronia melanocarpa) fruits grown in Korea were prepared with hot water and ethanol. The functionalcompounds, antioxidant activity, and cytotoxicity of the ethanol extract from aronia fruit (EA) and the water extract fromaronia fruit (WA) were analyzed. The contents of total polyphenol, total flavonoid, and total vitamin C of EA weresignificantly higher than those of WA. In the analysis of phenolic compounds using an HPLC, the rutin contents of EA andWA were 90.59 mg/100 g and 72.54 mg/100 g, respectively. The antioxidant activity was evaluated by DPPH and ABTSradical scavenging activity analysis and FRAP assay. EA showed a significantly higher antioxidant activity than WA in allthree analyses. The cytotoxicity to human lung cancer cells (A549) was analyzed using SRB assay. As a result of the SRBassay, the cytotoxicity of EA and WA to A549 cells at a concentration of 500 μg/mL was 68.99% and 54.25%, respectively,indicating that both EA and WA have high cytotoxicity to A549 cells. Therefore, the biological activity of aronia fruit extractswas verified through this study, and these extracts are expected to be used as functional food materials.

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