Anti-inflammatory Effect of Isolated Phenolics from Sambucussieboldianavar. pendula Leaves on Raw 264.7 Cells with Stimulated Lipopolysaccharide

초록

Phenolic compounds are well known to be among the major bioactive compounds found in various plant sources. It has been reported that Sambucus sieboldiana, found on Ulleung Island in Korea, contains phenolic compounds, suggesting potential health benefits. Thus, in this study, we determined the inflammatory activity of Sambucus sieboldiana with Raw 264.7 mouse macrophage cells. Phenolic compounds isolated from Sambucus sieboldiana were exposed to LPS-stimulated macrophages to determine whether an anti-inflammatory effect existed. The inflammation-associated protein expression, activity, and gene expression levels were measured by means of ELISA, a western blot analysis, and qPCR. Phenolic compounds from Sambucus sieboldiana inhibited the expression of iNOS, COX-2, and their downstream products PGE2 and NO, which are part of the NF-kB signaling pathway. The mRNA gene expression levels of pro-inflammatory cytokines, in this case TNFα, IL1β, IL6, MCP1, and PTGES2, were significantly decreased. These results showed that phenolic compounds from Sambucus sieboldiana have an anti-inflammatory effect, suggesting that Sambucus sieboldiana can potentially be used as an anti-inflammatory treatment, such as therapeutic and/or cosmetic products for the skin, particularly for skin inflammation. However, further in vivo studies using phenolics from Sambucus sieboldiana are needed to support the current findings.

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